Pyrus communis_d'Anjou

'd'Anjou' (Pyrus communis L.) is a short-necking variety of pears in the Pyrus , originated in Belgium, with a medium volume, egg-like appearance, an average weight of 270-285 g, and a diameter of about 80 mm. When harvested, the fruit is hard in texture and generally cannot be eaten directly; it usually needs to be cooked at room temperature 7-10 days after ripening. The ripe 'd'Anjou' pear fruit is soft and juicy, with few stone cells, good solubility, aroma and high quality. However, after ripening, the fruit becomes soft, is not resistant to storage and transportation, and has a short edible period.

We generated approximately 134 million paired-end reads from Illumina HiSeq and a total of 1,054,992 PacBio continuous long reads (CLR) with a read length N50 of 20 Kb, providing an estimated 67-fold and 21-fold coverage respectively of the expected 600 Mb Pyrus communis genome . Additionally, approximately 468 million 2 x 150 bp paired reads (~234-fold coverage) with an estimated mean molecule length (linked-reads) of 20 kb were generated using 10x Genomics Chromium Technology. The final meta-assembly, generated with a combination of the three datasets, contains 5,800 scaffolds with a N50 of 358 Kb.The total assembly size was 600Mb, and 45981 genes were predicted.

Sequencing Platforms                PacBio+Illumina+10x Genomics

 Assembly Level                         Chromosome

Assembled Genome Size           600Mb

Scaffold N50                              0.36Mb

Gene Number                             45981