Pyrus pyrifolia_Nijisseiki

 The Japanese pear variety ‘Nijisseiki’ (a Japanese term referring to the 20th century) was discovered in Matsudo (Chiba, Japan) in 1888. Due to its favourable flesh texture, ‘Nijisseiki’ was the leading Japanese pear variety in Japan from the 1940s to the 1980s, and ‘Nijisseiki’ was widely used as a breeding parent for the development of Japanese pear cultivars in Japan.

De novo assembly of PacBio Sequel long sequence reads covered 136× of the Japanese pear genome and generated 503.9 Mb contigs consisting of 114 sequences with an N50 value of 7.6 Mb. Contigs were assigned to Japanese pear genetic maps to establish 17 chromosome-scale sequences. In total, 44,876 protein-encoding genes were predicted, 84.3% of which were supported by predicted genes and transcriptome data from Japanese pear relatives. 

Variety                                       Nijisseiki

Sequencing Platforms               PacBio

 Assembly Level                       Chromosome

Estimated Genome Size           537.1Mb

Assembled Genome Size         504.1Mb

Scaffold N50                            31.6Mb

Contig N50                              7.6Mb

Gene Number                          44876